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112. OUTCOME OF IMMUNIZATION OF PATIENTS WITH AUTOLOGOUS GLIOBLASTOMA CELLS TRANSDUCED WITH HUMAN IFN-GAMMA GENE Leif G. Salford, 1 Eva Ask, 2 Cathrin Baurus Koch, 3 Anna Darabi, 1 Elisabeth Englund, 4 Shorena Janelidze, 1 Elna-Marie Larsson, 5 Bertil R.R. Persson, 3 Anna Rydelius, 2 Peter Siesj, 1 Gunnar Skagerberg, 1 Edward Visse, 1 and Bengt Widegren6; 1Department of Neurosurgery, 2Department of Neurology, 3Department of Medical Radiation Physics, 4Department of Neuropathology, 5Department of Neuroradiology, and 6Department of Tumour Immunology, The Rausing Laboratory, Lund University, Lund, Sweden Successful experimental immunogene tumor therapy in rats inoculated with glioma cells in the brain Visse et al., Cancer Gene Ther., 1999, 6: 3744 ; was our basis for translating the technique to patients with glioblastoma multiforme treated in the Department of Neurosurgery, Lund University Hospital, Lund, Sweden. Patients aged 50 to 69 years for whom 80% of the tumor can be surgically removed are included. The resected glioma cells are cultivated in a clinical transduction laboratory, and karyotyping excludes normal cells. By adenoviral vector technique, the human IFN- gene is transfected into the glioma cells, which, after irradiation, are injected intradermally in the arm. Each patient receives at least 4 and a maximum of 10 immunizations. The DTH reaction is measured at the injection sites. A week after injection, a skin biopsy is taken from the injection site. The cellular infiltration in the skin is studied by markers for T lymphocytes, helper cells, NK cells, and B lymphocytes. Expressions of cytokines for functional T-cell subsets are studied. Peripheral blood is sampled both before and after operation and after each immunization and is cocultured with the tumor cells, which allows for a selection of T-cells that can recognize tumor-specific antigens. The patients are followed with MRI and neuropsychological examination after every second immunization. We present both the clinical results for 6 fully treated patients and the patients' immunological responses, revealed by DTH and skin cellular infiltration. The analyzed cocultures of PBL and tumor cells are presented separately in an abstract by Siesj et al. Advertised before Acceptance under section 20 1 ; Proviso 1298974-July 27, 2004. CAPLIN POINT LABORATORIES LTD. 9, C WING, IV FLOOR, PARSN MANNERE, 602, ANNA SALAI, CHENNAI - 600 006. MANUFACTURERS & EXPORTERS. Proposed to be used. CHENNAI ; PHARMACEUTICAL AND MEDICINAL PREPRATIONS.

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